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@Nucleomics-VIB

VIB - Nucleomics Core

VIB | sequencing facility

VIB Nucleomics Core

Sequencing, analysis, and the code in between.

We run long- and short-read sequencing for the VIB research community and build the pipelines, toolboxes, and web tools that turn raw instrument output into answers. This organization holds that code — from one-line awk helpers to containerised Nextflow pipelines.

repos platforms licence


Start here

You almost certainly arrived holding data. Find the row that matches it.

You have… Start with Then reach for
PacBio Revio / Kinnex HiFi reads pacbio-tools Kinnex_16S_decat_demux_bashNC_HiFi-16S-workflow
Oxford Nanopore reads nanopore-tools ngs-tools
Element AVITI output aviti-tools variant_analysis
Full-length 16S amplicons NC_HiFi-16S-workflow benchmarks
Fungal / eukaryote ITS amplicons NC_NextITS
Bulk RNA-seq (BRB-seq) BRBseq-tools
A plot to make or an app to share plotting-tools Shiny-apps
A server to wrangle, files to move admin-tools NC_cloud-dl

How the code fits together

flowchart LR
  I["🧬 Instrument<br/>PacBio · ONT · AVITI · MGI"]
  P["Platform toolkits<br/><i>pacbio-tools · nanopore-tools<br/>aviti-tools · ngs-tools</i>"]
  A["Assay pipelines<br/><i>16S · ITS · exome · shotgun</i>"]
  V["Variants & genomes<br/><i>variant_analysis · ChimericSeq</i>"]
  R["Figures & apps<br/><i>plotting-tools · Shiny-apps</i>"]
  D["📦 Delivery to the researcher"]

  I --> P --> A --> R --> D
  P --> V --> R
  O["Core operations<br/><i>admin-tools · NC_cloud-dl</i>"] -.-> P
  O -.-> A
  O -.-> D
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Platform toolkits do the demultiplexing and QC that every project needs. Assay pipelines take it from there. Reporting code is deliberately separate, so the same figures can be regenerated years later.

Families

Each family has its own index with a repo-by-repo breakdown.

Family What lives there Index
🧪 Sequencing platform toolkits Per-instrument toolboxes: demultiplexing, QC, run parsing, format wrangling browse →
🦠 Amplicon & metabarcoding 16S, ITS and Kinnex pipelines from raw reads to taxonomy tables browse →
🧫 Genomes & variants Variant calling, assembly QC, chimera and transcript analysis browse →
📊 Visualization & reporting Publication-quality figures, interactive Shiny apps, method benchmarks browse →
⚙️ Core operations Sysadmin helpers, data movement, day-to-day glue browse →
🏛️ Legacy & reference Stable, still-cited, no longer actively developed browse →

Prefer to browse rather than be routed? All repositories on GitHub → — ordered by last push. Note that org-wide maintenance batches (licence and template sweeps) count as pushes, so recent dates there do not always mean recent work.

Reading a repo name

Our names are a convention, not an accident. Once you know the prefix, you know what you are looking at:

Pattern Meaning Example
*-tools A toolbox of many small, independent scripts for one platform or domain pacbio-tools
NC_* A Core-operated pipeline or application — production-facing, versioned NC_HiFi-16S-workflow
*_docker / *_nf A containerised or Nextflow implementation of a sibling pipeline NC_HiFi-16S-workflow_docker
dev_wt_* An internal web tool under development (internal)
wbt_* A deployed internal web tool (internal)

Not every name follows it. Repos that predate the convention (benchmarks, InSilico_PCR, Shiny-apps, variant_analysis, 16S_analysis_pipeline) and forks that keep their upstream name (ChimericSeq, mplotter) are left as they are on purpose — a rename leaves a silent GitHub redirect, so old clones keep working while quietly pointing somewhere else. Use the topic filters below rather than the prefix when you are unsure.

Filter by topic

Every repository carries topics on four axes. These are curated, not guessed — filtering on one gives you a real shortlist:

Axis Pick one
Platform pacbio · nanopore · aviti · mgi which instrument made the data
Assay 16s · its · amplicon · shotgun · rnaseq · assembly · variant-calling what was done to it
Shape pipeline · toolbox · container · shiny-app · visualization · benchmark what kind of thing it is
Lifecycle legacy stable, no longer developed

Language topics (bash, python, r, nextflow) are there too, but they describe how it is written rather than what it does.

shotgun and assembly link to a family page rather than a filter: the repos carrying those topics are internal, so the filter would return nothing to a visitor.

A note on what you can see. A good part of the Core's code is internal: LIMS-adjacent web tools, instrument dashboards, pricing calculators, and infrastructure that only makes sense inside our network. Those repos are private and deliberately absent from this page. Everything indexed here is public and usable outside VIB.

Using our code

Our code is licensed GPL-3.0: use it, adapt it, redistribute it — credit VIB Nucleomics Core and licence derived work under the same terms. Documentation and tutorial repos carry CC BY-SA 4.0 instead, and each repo's LICENSE is authoritative — the few repos forked from upstream projects keep the upstream licence. Everything was relicensed on 2026-08-27 from CC BY-SA 3.0, which no licence scanner could read and which Creative Commons does not recommend for source code; copies obtained before that date remain available under the old terms.

The code is written to be read, mostly Bash and R with comments rather than frameworks. A few caveats before you clone:

  • Pipelines assume our reference layout. Paths and reference genome locations are usually configurable at the top of the script; check there first.
  • Container images beat manual installs. Where a _docker sibling exists, use it.
  • Issues are welcome, including from outside VIB. We read them.

Credits

Created and maintained by Stephane PlaisanceVIB Nucleomics Core.

Contributions from the Core's bioinformatics and lab teams across the repos listed above.

Org profile v1.2.3 · 2026-08-27 · nucleomics.be

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  1. nanopore-tools nanopore-tools Public

    Scripts and utilities to process ONT data (MinION)

    Shell 4

  2. pacbio-tools pacbio-tools Public

    Tools for PacBio Sequel data analysis

    Shell 8 3

  3. plotting-tools plotting-tools Public

    Shareable code produced at the Core upon request or to support data re-analysis

    R

  4. hifi-16s-workflow-nc hifi-16s-workflow-nc Public

    Forked from PacificBiosciences/HiFi-16S-workflow

    Nextflow pipeline to analyze PacBio HiFi full-length 16S data

    Nextflow 1

  5. aviti-tools aviti-tools Public

    scripts and code for the Aviti data

    HTML 1

  6. nextits-nc nextits-nc Public

    Forked from vmikk/NextITS

    A pipeline for metabarcoding fungi and other eukaryotes with full-length ITS sequenced with PacBio

    HTML

Repositories

Showing 10 of 29 repositories

People

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Most used topics

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