Dynein Function Analysis

The Matlab code I wrote to compute the extent of the spindle poles by solving systems of linear Diophantine equations.

Quick start

This repository implements spindle-pole tracking and geometry analysis for the meiotic spindle in Matlab. See DEPENDENCIES.md for the Image Processing Toolbox requirement. Each script prompts interactively for its input image(s).

Repository contents

• EB3a.m -- EB3 comet feature detection.
• calculateRadii.m, eb1angles.m, track2Poles.m, track3Poles.m, trackPoles.m -- spindle-pole tracking and geometry, using lineIntersect.m to solve for pole positions via systems of linear Diophantine equations.
• fsmPrepScaleSpace.m -- a scale-space speckle-segmentation script.
• media/ -- an example video.
• License: see LICENSE -- research/educational use.

About

My work on the extent of the spindle poles by solving systems of linear Diophantine equations for the publications:

Ge Yang, Lisa Cameron, Paul Maddox, Ted Salmon, Gaudy Danuser "Regional Variation of Microtubule Flux Reveals Microtubule Organization in the Metaphase Meiotic Spindle" (2008) and Jay Gatlin, Alex Matov, Aaron Groen, Dan Needleman, Tom Maresca, Gaudy Danuser, Tim Mitchison, Ted Salmon "Spindle Fusion Requires Dynein-Mediated Sliding of Oppositely Oriented Microtubules" (2009).

For detailed information, see: https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1681065