diff --git a/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-main.yaml b/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-main.yaml
index 932cb1919a3be..f97393ad2c2d8 100644
--- a/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-main.yaml
+++ b/ci-operator/config/openshift/machine-api-operator/openshift-machine-api-operator-main.yaml
@@ -282,10 +282,20 @@ tests:
   steps:
     cluster_profile: vsphere-elastic
     env:
-      POOLS: vcenter.ci.ibmc.devcluster.openshift.com-cidatacenter-cicluster vcenter.ci.ibmc.devcluster.openshift.com-cidatacenter-1-cicluster-1
-        vcenter-1.ci.ibmc.devcluster.openshift.com-cidatacenter-2-cicluster-3
+      MAX_VCENTERS: "3"
       TEST_SKIPS: 'In-tree Volumes \[Driver: vsphere\]'
     workflow: openshift-e2e-vsphere-multi-vcenter
+- always_run: false
+  as: e2e-vsphere-ovn-multi-vcenter-serial
+  optional: true
+  steps:
+    cluster_profile: vsphere-elastic
+    env:
+      MAX_VCENTERS: "3"
+      TEST_SKIPS: ""
+      TEST_SUITE: openshift/conformance/serial
+    workflow: openshift-e2e-vsphere-multi-vcenter
+  timeout: 6h0m0s
 - always_run: false
   as: e2e-vsphere-operator
   optional: true
diff --git a/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-main-presubmits.yaml b/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-main-presubmits.yaml
index 334590123887f..17022df49b3e6 100644
--- a/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-main-presubmits.yaml
+++ b/ci-operator/jobs/openshift/machine-api-operator/openshift-machine-api-operator-main-presubmits.yaml
@@ -2301,6 +2301,111 @@ presubmits:
         secret:
           secretName: result-aggregator
     trigger: (?m)^/test( | .* )e2e-vsphere-ovn-multi-vcenter,?($|\s.*)
+  - agent: kubernetes
+    always_run: false
+    branches:
+    - ^main$
+    - ^main-
+    cluster: vsphere02
+    context: ci/prow/e2e-vsphere-ovn-multi-vcenter-serial
+    decorate: true
+    decoration_config:
+      sparse_checkout_files:
+      - .ci-operator.yaml
+      - Dockerfile.rhel
+      timeout: 6h0m0s
+    labels:
+      ci-operator.openshift.io/cloud: vsphere
+      ci-operator.openshift.io/cloud-cluster-profile: vsphere-elastic
+      ci.openshift.io/generator: prowgen
+      pj-rehearse.openshift.io/can-be-rehearsed: "true"
+    name: pull-ci-openshift-machine-api-operator-main-e2e-vsphere-ovn-multi-vcenter-serial
+    optional: true
+    rerun_command: /test e2e-vsphere-ovn-multi-vcenter-serial
+    spec:
+      containers:
+      - args:
+        - --enable-secrets-store-csi-driver=true
+        - --gcs-upload-secret=/secrets/gcs/service-account.json
+        - --gsm-config=/etc/gsm-config/gsm-config.yaml
+        - --gsm-credentials-file=/etc/gsm-credentials/key.json
+        - --gsm-project-config=/etc/gsm-config/gsm-project-config.yaml
+        - --image-import-pull-secret=/etc/pull-secret/.dockerconfigjson
+        - --lease-server-credentials-file=/etc/boskos/credentials
+        - --report-credentials-file=/etc/report/credentials
+        - --secret-dir=/secrets/ci-pull-credentials
+        - --target=e2e-vsphere-ovn-multi-vcenter-serial
+        command:
+        - ci-operator
+        env:
+        - name: HTTP_SERVER_IP
+          valueFrom:
+            fieldRef:
+              fieldPath: status.podIP
+        image: quay-proxy.ci.openshift.org/openshift/ci:ci_ci-operator_latest
+        imagePullPolicy: Always
+        name: ""
+        ports:
+        - containerPort: 8080
+          name: http
+        resources:
+          requests:
+            cpu: 10m
+        volumeMounts:
+        - mountPath: /etc/boskos
+          name: boskos
+          readOnly: true
+        - mountPath: /secrets/ci-pull-credentials
+          name: ci-pull-credentials
+          readOnly: true
+        - mountPath: /secrets/gcs
+          name: gcs-credentials
+          readOnly: true
+        - mountPath: /etc/gsm-config
+          name: gsm-config
+          readOnly: true
+        - mountPath: /etc/gsm-credentials
+          name: gsm-sa-key
+          readOnly: true
+        - mountPath: /secrets/manifest-tool
+          name: manifest-tool-local-pusher
+          readOnly: true
+        - mountPath: /etc/pull-secret
+          name: pull-secret
+          readOnly: true
+        - mountPath: /etc/report
+          name: result-aggregator
+          readOnly: true
+      serviceAccountName: ci-operator
+      volumes:
+      - name: boskos
+        secret:
+          items:
+          - key: credentials
+            path: credentials
+          secretName: boskos-credentials
+      - name: ci-pull-credentials
+        secret:
+          secretName: ci-pull-credentials
+      - configMap:
+          name: gsm-config
+        name: gsm-config
+      - csi:
+          driver: secrets-store.csi.k8s.io
+          readOnly: true
+          volumeAttributes:
+            secretProviderClass: ci-operator-sa-key-spc
+        name: gsm-sa-key
+      - name: manifest-tool-local-pusher
+        secret:
+          secretName: manifest-tool-local-pusher
+      - name: pull-secret
+        secret:
+          secretName: registry-pull-credentials
+      - name: result-aggregator
+        secret:
+          secretName: result-aggregator
+    trigger: (?m)^/test( | .* )e2e-vsphere-ovn-multi-vcenter-serial,?($|\s.*)
   - agent: kubernetes
     always_run: false
     branches: