diff --git a/src/api/hooks.cc b/src/api/hooks.cc
index 09a79fb9da81..b46073b6b7cf 100644
--- a/src/api/hooks.cc
+++ b/src/api/hooks.cc
@@ -145,8 +145,15 @@ static ExclusiveAccess<CleanupHookRegistry> cleanup_hook_registry;
 
 static void CleanupHookThunkRun(void* arg) {
   const CleanupHookThunk* thunk = static_cast<CleanupHookThunk*>(arg);
-  thunk->fun(thunk->arg);
-  RemoveEnvironmentCleanupHook(thunk->isolate, thunk->fun, thunk->arg);
+  // `thunk->fun` may itself remove and free this CleanupHookThunk (e.g. via
+  // ~ObjectWrap(), which calls RemoveEnvironmentCleanupHook()), so cache the
+  // fields we still need before invoking it rather than reading them from
+  // `thunk` afterwards.
+  Isolate* isolate = thunk->isolate;
+  CleanupHook fun = thunk->fun;
+  void* fun_arg = thunk->arg;
+  fun(fun_arg);
+  RemoveEnvironmentCleanupHook(isolate, fun, fun_arg);
 }
 
 void AddEnvironmentCleanupHook(Isolate* isolate,
diff --git a/test/cctest/test_environment.cc b/test/cctest/test_environment.cc
index 59c71835499e..9203c4f8d713 100644
--- a/test/cctest/test_environment.cc
+++ b/test/cctest/test_environment.cc
@@ -28,6 +28,12 @@ static void at_exit_callback_ordered2(void* arg);
 static void at_exit_js(void* arg);
 static std::string cb_1_arg;  // NOLINT(runtime/string)
 
+struct SelfRemovingCleanupHookState {
+  v8::Isolate* isolate;
+  bool ran = false;
+};
+static void self_removing_cleanup_hook(void* arg);
+
 class EnvironmentTest : public EnvironmentTestFixture {
  private:
   void TearDown() override {
@@ -289,6 +295,26 @@ TEST_F(EnvironmentTest, AtExitRunsJS) {
   EXPECT_TRUE(called_at_exit_js);
 }
 
+// A cleanup hook that removes itself while the environment cleanup queue is
+// being drained must not cause a use-after-free. Since #63642 this is the
+// ordinary teardown path for every node::ObjectWrap still alive at exit.
+// The use-after-free is silent in ordinary builds; it is caught by the
+// ASan/Valgrind CI, which is also how the original assertion (#63923)
+// surfaced. Regression test for https://github.com/nodejs/node/issues/65195.
+TEST_F(EnvironmentTest, RemoveEnvironmentCleanupHookDuringCleanup) {
+  const v8::HandleScope handle_scope(isolate_);
+  const Argv argv;
+  SelfRemovingCleanupHookState state{isolate_};
+  {
+    Env env{handle_scope, argv};
+    node::AddEnvironmentCleanupHook(
+        isolate_, self_removing_cleanup_hook, &state);
+    // Destroying `env` runs FreeEnvironment() -> RunCleanup(), which drains
+    // the cleanup queue and invokes CleanupHookThunkRun() for the hook above.
+  }
+  EXPECT_TRUE(state.ran);
+}
+
 TEST_F(EnvironmentTest, MultipleEnvironmentsPerIsolate) {
   const v8::HandleScope handle_scope(isolate_);
   const Argv argv;
@@ -372,6 +398,19 @@ static void at_exit_js(void* arg) {
   called_at_exit_js = true;
 }
 
+// Mirrors what node::ObjectWrap does since
+// https://github.com/nodejs/node/pull/63642: the destructor removes the
+// object's own environment cleanup hook. When that runs while the cleanup
+// queue is being drained, CleanupHookThunkRun() must not read the
+// CleanupHookThunk after invoking the hook -- the hook has already erased and
+// freed it. See https://github.com/nodejs/node/issues/65195.
+static void self_removing_cleanup_hook(void* arg) {
+  auto* state = static_cast<SelfRemovingCleanupHookState*>(arg);
+  state->ran = true;
+  node::RemoveEnvironmentCleanupHook(
+      state->isolate, self_removing_cleanup_hook, state);
+}
+
 TEST_F(EnvironmentTest, SetImmediateCleanup) {
   int called = 0;
   int called_unref = 0;